National Standard Methods in PDF Format

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Title

Status

Issue No

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BSOPID 1

Introduction to the preliminary identification of medically important bacteria

I

1.4

Feb 08

BSOPID 2

Identification of Corynebacterium species

I

3

Jul 08

BSOPID  3

Identification of Listeria species, non-toxigenic Corynebacterium species and other non-sporing gram-positive rods

I

2

Oct 07

BSOPID 4

Identification of Streptococcus species, Enterococcus species and morphologically similar organisms

I

2.1

Sep 07

BSOPID 5

Identification of Bordetella pertussis and Bordetella parapertussis from selective agar

I

2.1

Sep 07

BSOPID 6

Identification of Neisseria species

UR

N/A

N/A

BSOPID 7

Identification of Staphylococcus species, Micrococcus species and Rothia species

I

2.1

Sep 07

BSOPID 8

Identification of Clostridium species

I

3

Jul 08

BSOPID 9

Identification of Bacillus species

I

2.1

Sep 07

BSOPID 10

Identification of aerobic Actinomycetes species

D

N/A

N/A

BSOPID 11

Identification of Moraxella species and morphologically similar organisms

I

2

Aug 07

BSOPID 12

Identification of Haemophilus species and the HACEK group of organisms

UR

N/A

N/A

BSOPID 13

Identification of Pasteurella species and morphologically similar bacteria

I

2.1

Sep 07

BSOPID 14

Identification of non-sporing, non-branching anaerobes

D

N/A

N/A

BSOPID 15

Identification of anaerobic Actinomycetes species

D

N/A

N/A

BSOPID 16

Identification of Enterobacteriaceae

I

2

Nov 07

BSOPID 17

Identification of glucose non-fermenting Gram-negative rods

I

2

Apr 07

BSOPID 18

Identification of Legionella species

I

2

Nov 07

BSOPID 19

Identification of Vibrio species

I

2

Nov 07

BSOPID 20

Identification of Shigella species

I

2

Nov 07

BSOPID 21

Identification of Yersinia species from faeces

I

2

Nov 07

BSOPID 22

Identification of Escherichia coli O157

I

2

Nov 07

BSOPID 23

Identification of Campylobacter species

I

2

Nov 07

BSOPID 24

Identification of Salmonella species

I

2.1

Sep 08

BSOPID 25

Identification of anaerobic Gram-negative rods

D

N/A

N/A

BSOPID 26

Identification of Helicobacter species

I

2

Feb 08

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Status

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Issue Date

F 2

Preparation of Samples and Dilutions

I

1.4

May 05

F 10

Aerobic Plate Count at 30°C: Surface Plate Method

I

1.4

May 05

F 11

Aerobic Plate Count at 30°C: Spiral Plate Method

I

1.4

May 05

F 12

Enumeration of Staphylococcus aureus

I

1.4

May 05

F 13

Detection of Salmonella species

I

3.1

Jul 08

F 14

Enumeration of Clostridium perfringens

I

2.4

May 05

F 15

Enumeration of Bacillus cereus and other Bacillus species

I

1.4

May 05

F 16

Enumeration of Escherichia coli in Raw Molluscs by the Multiple Tube Technique

I

4.2

May 05

F 17

Detection of Escherichia coli O157 by Automated Immunomagnetic Bead Separation

I

3

May 08

F 18

Detection and Enumeration of Enterobacteriaceae

I

1

Dec 05

F 19

Detection and Enumeration of Listeria monoctyogenes & other Listeria species

I

2

Dec 07

F 20

Direct Enumeration of Escherichia coli

I

1.4

May 05

F 21

Detection of Campylobacter species

I

2.1

Aug 08

F 22

Enumeration of ß-glucuronidase positive Escherichia coli - Most Probable Number method

I

1

Dec 05

F 23

Enumeration of Enterobacteriaceae by the Colony Count Technique

I

1.4

May 05

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Status

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BSOPTP 1

Control organisms for National Standard Method BSOP Test Procedures

D

N/A

N/A

BSOPTP 2

Aesculin hydrolysis test

I

1.1

May 05

BSOPTP  3

Agglutination test

UR

1.1

May 05

BSOPTP 5

Bile solubility test

UR

1.1

May 05

BSOPTP 8

Catalase test

UR

1.2

Jun 06

BSOPTP 10

Coagulase test

UR

3

Oct 05

BSOPTP 12

Deoxyribonuclease test

UR

1.1

May 05

BSOPTP 19

Indole test

UR

1.1

May 05

BSOPTP 21

Motility test

UR

1.1

May 05

BSOPTP 22

Nagler test

UR

1.1

May 05

BSOPTP 24

ONPG (ß-galactosidase) test

UR

1.1

May 05

BSOPTP 25

Optochin test

UR

N/A

N/A

BSOPTP 26

Oxidase test

UR

N/A

N/A

BSOPTP 27

Oxidation and fermentation of glucose test

UR

N/A

N/A

BSOPTP 29

Porphyrin synthesis (ala) test

UR

N/A

N/A

BSOPTP 30

Potassium hydroxide test

UR

N/A

N/A

BSOPTP 32

Changing the phase of Salmonella test

UR

N/A

N/A

BSOPTP 34

Thermonuclease activity test

UR

N/A

N/A

BSOPTP 36

Urease test

UR

N/A

N/A

BSOPTP 38

X and V factor test

UR

N/A

N/A

BSOPTP 39

Staining Procedures

I

1

Mar 07

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Title

Status

Issue No

Issue Date

D 1

Preparation of samples and decimal dilutions

I

2.4

May 05

D 2

Plate count test at 30°C

I

3.1

May 05

D 3

Plate count test at 21°C (pre-incubated)

I

3.1

May 05

D 4

Enumeration of coliforms - colony count at 30°C

I

2.4

May 05

D 5

Enumeration of coliforms and presumptive Escherichia coli by the most probable number (MPN) technique

I

2.4

May 05

D 6

Determination of peroxidase activity (Storch test) in milk

I

2.4

May 05

D 7

Determination of alkaline Phosphatase Activity in Pasteurised Milk and Cream - Fluorimetric Method

I

1.4

May 05

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Title

Status

Issue No

Issue Date

VSOP 2

Gastroenteritis: sporadic cases

I

5

Jul 07

VSOP 3

Gastroenteritis: Outbreaks

I

6

Aug 07

VSOP 4

Hepatitis B diagnostic serology in the immunocompetent (including Hepatitis B in pregnancy

UR / I

4.1

June 03

VSOP 5

Investigation of Hepatitis C Infection

UR

N/A

N/A

VSOP 6

Hepatitis, jaundice and abnormal LFTs

I

2.2

Nov 05

VSOP 8

Vertical HCV transmission algorithm

I

1

Nov 07

VSOP  10

Blood borne virus testing in dialysis patients

I

1

Feb 08

VSOP 11

Anti-HIV Screening

UR

N/A

N/A

VSOP 12

Preparation of coated grids for electron microscopy

I

5.1

Aug 06

VSOP 13

Electron Microscopy: Flotation (direct) method

I

5

Feb 07

VSOP 14

Electron Microscopy: Differential Centrifugation (double-spin method)

I

5

Feb 07

VSOP 15

Electron microscopy: ammonium sulphate precipitation

I

5

Sep 07

VSOP 16

Investigation of clinical specimens by electron microscopy using solid phase immune electron microscopy

I

5

Sep 07

VSOP 17

Isolation of herpes simplex virus associated with herpes genitalis

I

4

Jul 07

VSOP 18

Complement fixation tests

I

2

Jul 06

VSOP 20

Nucleic acid extraction by the Boom method

WD

N/A

N/A

VSOP 21

Isolation of Viruses associated with infections of the eye: Keratoconjunctivitis

I

2

Feb 07

VSOP 22

Immunofluorescence and isolation of viruses from respiratory samples

I

1

Jul 07

VSOP 23

Investigation of human herpes viruses (excluding herpes genitalis)

I

2

Feb 06

VSOP 24

Isolation of enteroviruses and parechoviruses

I

2

May 06

VSOP 25

Real-Time Quadriplex PCR for the detection of Influenza

C

1

Nov 06

VSOP 26

Epstein-Barr Virus serology (formerly VSOP 6.2)

I

3

Mar 08

VSOP 27

Hepatitis A virus acute infection serology (formerly VSOP6.3)

I

2.4

Oct 07

VSOP 28

Cytomegalovirus serology (formerly VSOP6.4)

I

2.4

Oct 07

VSOP 30

Investigation of erythrovirus (parvovirus) B19 in pregnant women exposed to rash illness

UR

N/A

N/A

VSOP 31

Management of confirmed erythrovirus (parvovirus) B19 infection in pregnancy

UR

N/A

N/A

VSOP 32

Investigation of rubella in pregnant women of unknown rubella immunity status exposed to rash illness

UR

N/A

N/A

VSOP 33

Pregnant patient in contact with rash illness

I

2.2

May 05

VSOP 34

Investigation of pregnant patient with rash illness

I

2.2

May 05

VSOP 35

Investigation of Cytomegalovirus infection by Roche Lightcycler PCR

I

2.1

Aug 07

VSOP 36

Operation of the Roche MagNA Pure LC automated nucleic acid extraction robot

I

2

May 06

VSOP 37

Chlamydia Infection - Testing by Nucleic Acid Amplification Test (NAATs)

I

2.1

Sep 08

VSOP 38

Detection of SARS-CoV by reverse transcription PCR

C

1.2

May 05

VSOP 39

Procedure for the care and propagation of cell cultures for virus isolation

I

1

May 06

VSOP 40

RT-PCR (Block Based) for Influenza H5 viruses

I

3.3

Nov 06

VSOP 41

Real-Time RT-PCR (Taq Man) for influenza A H5 viruses

I

4.1

May 06

VSOP 42

RT-PCR for the detection of influenza A viruses

I

1.3

May 06

VSOP 43

Investigation of viral encephalitis

UR

N/A

N/A

VSOP 44

Serological Diagnosis of Syphilis

I

1

Nov 07

VSOP 45

Haemadsorption of viruses

I

1

Aug 06

VSOP 46

European Real-Time RT-PCR for Influenza A H5 viruses

I

1.1

May 06

Ref No

Title

Status

Issue No

Issue Date

W 1

General technique for the detection and enumeration of bacteria by negative pressure membrane filtration

I

4.1

Apr 07

W 2

Enumeration of coliform bacteria and Escherichia coli by membrane filtration

I

4.1

Oct 07

W 3

Enumeration of enterococci by membrane filtration

I

3

Aug 06

W 4

Aerobic colony count by the pour plate method

I

4.2

Aug 08

W 5

Enumeration of Clostridium perfringens by membrane filtration

I

3.1

May 05

W 6

Enumeration of Pseudomonas aeruginosa by membrane filtration

I

3

Dec 07

W 7

Detection of Salmonella species

I

3

Jul 06

W 8

Detection of Campylobacter species in water

I

3

Oct 07

W 9

Enumeration of Aeromonas species by membrane filtration

I

1.3

May 05

W 10

Enumeration of Staphylococcus aureus by membrane filtration

I

3.3

May 05

W 12

Detection and enumeration of Legionella species by filtration and centrifugation

I

1.2

Aug 06

W 13

Detection and enumeration of Legionella species by centrifugation

I

1.2

Aug 06

W 14

Detection and enumeration of Legionella species by positive pressure membrane filtration

I

1.2

Aug 06

W 15

Detection and enumeration of Legionella species in biofilms and sediments

I

1.2

Aug 06

W 16

Detection of Escherichia coli O157 by automated immunomagnetic separation

I

4

Mar 06

W 17

Isolation and identification of Acanthamoeba species

I

2.3

May 05

W 18

Enumeration of coliforms and Escherichia coli by Idexx (Colilert 18) Quanti-TrayTM

I

2.3

May 05

W 22

Enumeration of viable micro-organisms: Aerobic colony count by membrane filtration method

I

1

Dec 07