National Standard Methods in PDF Format

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Title

Status

Issue No

Issue Date

BSOPID 1

Introduction to the preliminary identification of medically important bacteria

I

1.4

Feb 08

BSOPID 10

Identification of aerobic Actinomycetes

I

1

Nov 09

BSOPID 11

Identification of Moraxella species and morphologically similar organisms

I

2

Aug 07

BSOPID 12

Identification of Haemophilus species and the HACEK group of organisms

I

2

Apr 09

BSOPID 13

Identification of Pasteurella species and morphologically similar bacteria

I

2.1

Sep 07

BSOPID 14

Identification of anaerobic cocci

I

2

Dec 09

BSOPID 15

Identification of anaerobic Actinomycetes species

I

1

Dec 09

BSOPID 16

Identification of Enterobacteriaceae

I

2

Nov 07

BSOPID 17

Identification of glucose non-fermenting Gram-negative rods

I

2

Apr 07

BSOPID 18

Identification of Legionella species

I

2

Nov 07

BSOPID 19

Identification of Vibrio species

I

2

Nov 07

BSOPID 2

Identification of Corynebacterium species

I

3

Jul 08

BSOPID 20

Identification of Shigella species

I

2

Nov 07

BSOPID 21

Identification of Yersinia species from faeces

I

2

Nov 07

BSOPID 22

Identification of Escherichia coli O157

I

2

Nov 07

BSOPID 23

Identification of Campylobacter species

I

2

Nov 07

BSOPID 24

Identification of Salmonella species

I

2.1

Sep 08

BSOPID 25

Identification of anaerobic Gram-negative rods

I

1

Dec 09

BSOPID 26

Identification of Helicobacter species

I

2

Feb 08

BSOPID  3

Identification of Listeria species, non-toxigenic Corynebacterium species and other non-sporing gram-positive rods

I

2

Oct 07

BSOPID 4

Identification of Streptococcus species, Enterococcus species and morphologically similar organisms

I

2.1

Sep 07

BSOPID 5

Identification of Bordetella pertussis and Bordetella parapertussis from selective agar

I

2.1

Sep 07

BSOPID 6

Identification of Neisseria species

UR

2.1

Sep 07

BSOPID 6.db

Identification of Neisseria species

UC

N/A

N/A

BSOPID 7

Identification of Staphylococcus species, Micrococcus species and Rothia species

I

2.1

Sep 07

BSOPID 8

Identification of Clostridium species

I

3

Jul 08

BSOPID 9

Identification of Bacillus species

I

2.1

Sep 07

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Status

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Issue Date

F 2

Preparation of Samples and Dilutions

I

1.4

May 05

F 10

Aerobic Plate Count at 30°C: Surface Plate Method

I

1.4

May 05

F 11

Aerobic Plate Count at 30°C: Spiral Plate Method

I

1.4

May 05

F 12

Enumeration of Staphylococcus aureus

I

1.4

May 05

F 13

Detection of Salmonella species

I

3.1

Jul 08

F 14

Enumeration of Clostridium perfringens

I

2.4

May 05

F 15

Enumeration of Bacillus cereus and other Bacillus species

I

1.4

May 05

F 16

Enumeration of Escherichia coli in Raw Molluscs by the Multiple Tube Technique

I

4.2

May 05

F 17

Detection of Escherichia coli O157 by Automated Immunomagnetic Bead Separation

I

3

May 08

F 18

Detection and Enumeration of Enterobacteriaceae

I

1

Dec 05

F 19

Detection and Enumeration of Listeria monoctyogenes & other Listeria species

I

3.1

Jan 09

F 20

Direct Enumeration of Escherichia coli

I

1.4

May 05

F 21

Detection of Campylobacter species

I

2.1

Aug 08

F 22

Enumeration of ß-glucuronidase positive Escherichia coli - Most Probable Number method

I

1

Dec 05

F 23

Enumeration of Enterobacteriaceae by the Colony Count Technique

I

1.4

May 05

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Title

Status

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BSOPTP 1

Control organisms for National Standard Method BSOP Test Procedures

D

N/A

N/A

BSOPTP 10

Coagulase test

I

4

Mar 10

BSOPTP 12

Deoxyribonuclease test

UR

1.1

May 05

BSOPTP 12.db

Deoxyribonuclease test

UC

N/A

N/A

BSOPTP 19

Indole test

UR

1.1

May 05

BSOPTP 19.dc

Indole test

UC

N/A

N/A

BSOPTP 2

Aesculin hydrolysis test

I

2

Mar 10

BSOPTP 21

Motility test

UR

1.1

May 05

BSOPTP 21.db

Motility test

UC

N/A

N/A

BSOPTP 22

Nagler test

UR

1.1

May 05

BSOPTP 22.db

Nagler test

UC

N/A

N/A

BSOPTP 24

ONPG (ß-galactosidase) test

UR

1.1

May 05

BSOPTP 24.dc

ONPG (ß-galactosidase) test

UC

N/A

N/A

BSOPTP 25

Optochin test

UR

1.1

May 05

BSOPTP 25.db

Optochin test

UC

N/A

N/A

BSOPTP 26

Oxidase test

UR

1.1

May 05

BSOPTP 26.dd

Oxidase test

UC

N/A

N/A

BSOPTP 27

Oxidation and fermentation of glucose test

UR

1.1

May 05

BSOPTP 27db

Oxidation and fermentation of glucose test

UC

N/A

N/A

BSOPTP 29

Porphyrin synthesis (ala) test

UR

1.1

May 05

BSOPTP 29.db

Porphyrin synthesis (ala) test

UC

N/A

N/A

BSOPTP  3

Agglutination test

I

2

Mar 10

BSOPTP 30

Potassium hydroxide test

UR

1.1

May 05

BSOPTP 30.da

Potassium hydroxide test

UC

N/A

N/A

BSOPTP 32

Changing the phase of Salmonella

UR

1.1

May 05

BSOPTP 32.dc

Changing the phase of Salmonella

UC

N/A

N/A

BSOPTP 34

Thermonuclease test

UR

1.1

May 05

BSOPTP 34.db

Thermonuclease test

UC

N/A

N/A

BSOPTP 36

Urease test

UR

1.1

May 05

BSOPTP 36.dc

Urease test

UC

N/A

N/A

BSOPTP 38

X and V factor test

UR

1.1

May 05

BSOPTP 38.dd

X and V factor test

UC

N/A

N/A

BSOPTP 39

Staining Procedures

I

1

Mar 07

BSOPTP 5

Bile solubility test

UR

1.1

May 05

BSOPTP 5.db

Bile solubility test

UC

N/A

N/A

BSOPTP 8

Catalase test

I

2

Mar 10

Ref No

Title

Status

Issue No

Issue Date

D 1

Preparation of samples and decimal dilutions

I

2.4

May 05

D 2

Plate count test at 30°C

I

3.1

May 05

D 3

Plate count test at 21°C (pre-incubated)

I

3.1

May 05

D 4

Enumeration of coliforms - colony count at 30°C

I

2.4

May 05

D 5

Enumeration of coliforms and presumptive Escherichia coli by the most probable number (MPN) technique

I

2.4

May 05

D 6

Determination of peroxidase activity (Storch test) in milk

I

2.4

May 05

D 7

Determination of alkaline Phosphatase Activity in Pasteurised Milk and Cream - Fluorimetric Method

I

1.4

May 05

Ref No

Title

Status

Issue No

Issue Date

VSOP  10

Blood borne virus testing in dialysis patients

I

1

Feb 08

VSOP 11

Anti-HIV Screening

I

2

Aug 09

VSOP 12

Preparation of coated grids for electron microscopy

I

6

Mar 10

VSOP 13

Electron Microscopy: Flotation (direct) method

UR

5

Feb 07

VSOP 13.da

Electron Microscopy: Flotation (direct) method

UC

N/A

N/A

VSOP 14

Electron Microscopy: Differential Centrifugation (double-spin method)

UR

5

Feb 07

VSOP 14.da

Electron Microscopy: Differential Centrifugation (double-spin method)

UC

N/A

N/A

VSOP 15

Electron microscopy: ammonium sulphate precipitation

I

5

Sep 07

VSOP 16

Investigation of clinical specimens by electron microscopy using solid phase immune electron microscopy

I

5

Sep 07

VSOP 17

Isolation of herpes simplex virus associated with herpes genitalis

I

4

Jul 07

VSOP 18

Complement fixation tests

1

3

Dec 09

VSOP 2

Gastroenteritis: sporadic cases

I

5

Jul 07

VSOP 20

Nucleic acid extraction by the Boom method

WD

N/A

N/A

VSOP 21

Isolation of Viruses associated with infections of the eye: Keratoconjunctivitis

UR

2

Feb 07

VSOP 21.db

Isolation of Viruses associated with infections of the eye: Keratoconjunctivitis

UC

N/A

N/A

VSOP 22

Immunofluorescence and isolation of viruses from respiratory samples

I

1

Jul 07

VSOP 23

Investigation of human herpes viruses (excluding herpes genitalis)

I

3

Nov 09

VSOP 24

Isolation of enteroviruses and parechoviruses

I

3

Nov 09

VSOP 25

Real-Time Quadriplex PCR for the detection of Influenza

I

1

Nov 06

VSOP 26

Epstein-Barr Virus serology (formerly VSOP 6.2)

I

3

Mar 08

VSOP 27

Hepatitis A virus acute infection serology (formerly VSOP6.3)

I

2.4

Oct 07

VSOP 28

Cytomegalovirus serology (formerly VSOP6.4)

I

2.4

Oct 07

VSOP 29

Swine-lineage Influenza A H1 specific fast Real Time PCR

C

2

May 09

VSOP 3

Gastroenteritis: Outbreaks

I

6

Aug 07

VSOP 30

Investigation of pregnant women exposed to rash illnesses (now incorporating VSOPs 31, 32, 33 and 34)

I

4

May 09

VSOP 31

Management of confirmed erythrovirus (parvovirus) B19 infection in pregnancy (now incorporated in VSOP 30)

WD

N/A

N/A

VSOP 32

Investigation of rubella in pregnant women of unknown rubella immunity status exposed to rash illness (now incorporated in VSOP 30)

WD

N/A

N/A

VSOP 33

Pregnant patient in contact with rash illness (now incorporated in VSOP 30)

WD

N/A

N/A

VSOP 34

Investigation of pregnant patient with rash illness (now incorporated in VSOP 30)

WD

N/A

N/A

VSOP 35

Investigation of Cytomegalovirus infection by Roche Lightcycler PCR

I

2.1

Aug 07

VSOP 36

Operation of the Roche MagNA Pure LC automated nucleic acid extraction robot

WD

N/A

N/A

VSOP 37

Chlamydia trachomatis Infection - Testing by Nucleic Acid Amplification Test (NAATs)

I

2.1

Sep 08

GP Ref. Guide 37.rg

Diagnosis of Chlamydia - Quick Reference Guide for General Practices

N/A

N/A

N/A

VSOP 38

Detection of SARS-CoV by reverse transcription PCR

I

2

May 09

VSOP 39

Procedure for the care and propagation of cell cultures for virus isolation

I

2

Dec 09

VSOP 4

Hepatitis B diagnostic serology in the immunocompetent (including Hepatitis B in pregnancy

UR

4.2

Jun 05

VSOP 4.dt

Hepatitis B diagnostic serology in the immunocompetent (including Hepatitis B in pregnancy

UC

N/A

N/A

VSOP 40

RT-PCR (Block Based) for Influenza H5 viruses

I

4

May 09

VSOP 41

Real-Time RT-PCR (Taq Man) for influenza A H5 viruses

I

5

May 09

VSOP 42

RT-PCR for the detection of influenza A viruses

I

2

May 09

VSOP 43

Investigation of viral encephalitis

I

2

Dec 09

VSOP 44

Serological Diagnosis of Syphilis

I

1

Nov 07

VSOP 45

Haemadsorption of viruses

I

2

Mar 10

VSOP 46

European Real-Time RT-PCR for Influenza A H5 viruses

I

2

May 09

VSOP 47

Investigation of Swine-Lineage Influenza A (H1N1) by PCR

I

3

Jun 09

VSOP 48

Swine-lineage Influenza A N1 Fast Real Time Confirmatory PCR assay

C

1

May 09

VSOP 49

Investigation of Swine-Lineage Influenza A/H1N1 v using the Quadriplex Influenza A PCR assay with Swine-Lineage Influenza A H1 and N1 assays

I

1.1

Jun 09

VSOP 5

Investigation of Hepatitis C Infection

UR

5

Aug 05

VSOP 5.dn

Investigation of Hepatitis C Infection

UC

N/A

N/A

VSOP 50

One-step influenza multiplex Real-Time RT-PCR

C

1

Jun 09

VSOP 51

Investigation of Swine-lineage Influenza A/H1N1v using the Swine-lineage Influenza A H1 assay

I

1

Jul 09

VSOP 6

Hepatitis, jaundice and abnormal LFTs

I

2.2

Nov 05

VSOP 8

Vertical HCV transmission algorithm

I

1

Nov 07

Ref No

Title

Status

Issue No

Issue Date

W 1

General technique for the detection and enumeration of bacteria by negative pressure membrane filtration

I

4.1

Apr 07

W 2

Enumeration of coliform bacteria and Escherichia coli by membrane filtration

I

4.1

Oct 07

W 3

Enumeration of enterococci by membrane filtration

I

3

Aug 06

W 4

Aerobic colony count by the pour plate method

I

4.2

Aug 08

W 5

Enumeration of Clostridium perfringens by membrane filtration

I

3.1

May 05

W 6

Enumeration of Pseudomonas aeruginosa by membrane filtration

I

3

Dec 07

W 7

Detection of Salmonella species

I

3

Jul 06

W 8

Detection of Campylobacter species in water

I

3

Oct 07

W 9

Enumeration of Aeromonas species by membrane filtration

I

1.3

May 05

W 10

Enumeration of Staphylococcus aureus by membrane filtration

I

3.3

May 05

W 12

Detection and enumeration of Legionella species by filtration and centrifugation

I

1.2

Aug 06

W 13

Detection and enumeration of Legionella species by centrifugation

I

1.2

Aug 06

W 14

Detection and enumeration of Legionella species by positive pressure membrane filtration

I

1.2

Aug 06

W 15

Detection and enumeration of Legionella species in biofilms and sediments

I

1.2

Aug 06

W 16

Detection of Escherichia coli O157 by automated immunomagnetic separation

I

4

Mar 06

W 17

Isolation and identification of Acanthamoeba species

I

2.3

May 05

W 18

Enumeration of coliforms and Escherichia coli by Idexx (Colilert 18) Quanti-TrayTM

I

2.3

May 05

W 22

Enumeration of viable micro-organisms: Aerobic colony count by membrane filtration method

I

1

Dec 07

Ref No

Title

Status

Issue No

Issue Date

S 

Acute Infective Hepatitis

I

1

Feb 10

S 

Conjunctivitis

I

1

Feb 10